Fluorescence patterns of chromatin and cytoplasm by hematoxylin solutions.

Treatments of chicken blood and Ehrlich ascites tumor smears with hematoxylin solutions give a fluorescence reaction in chromatin, basophilic cytoplasm and leukocyte granules. In these structures the fluorescence emission increases upon dye aging and prolonged staining times. We present a preliminary spectral analysis suggesting the possibility to employ hematoxylin as a fluorochrome.