Espelosin R H, Stockert J C
Z Naturforsch C Biosci. 1982 Jan-Feb;37(1-2):139-41. doi: 10.1515/znc-1982-1-226.
After treatment with neutral red at low concentrations (10(-6), 10(-5) M), the chromatin of chicken erythrocytes shows an intensive red fluorescence, which is reduced or practically abolished when nuclei become stained by using the dye at concentrations higher than 10(-4) M. Both the fluorescence and staining reactions are dependent on the DNA content of chromatin. Neutral red fluorescence of nuclei increases considerably after treatment with inorganic cations (Al3+, Ba2+), while previous treatments with methylene blue reduce the fluorescence intensity. The possibility that chromatin fluorescence depends on the intercalative binding of neutral red is suggested.
用低浓度（10⁻⁶、10⁻⁵ M）中性红处理后，鸡红细胞染色质呈现强烈的红色荧光，而当使用高于10⁻⁴ M浓度的染料使细胞核染色时，这种荧光会减弱或几乎消失。荧光和染色反应均取决于染色质的DNA含量。用无机阳离子（Al³⁺、Ba²⁺）处理后，细胞核的中性红荧光显著增加，而先前用亚甲蓝处理会降低荧光强度。这表明染色质荧光可能取决于中性红的嵌入结合。
