Smit N, Le Poole I, van den Wijngaard R, Tigges A, Westerhof W, Das P
Department of Dermatology, University of Amsterdam, The Netherlands.
Arch Dermatol Res. 1993;285(6):356-65. doi: 10.1007/BF00371837.
The expression of different immunological markers by cultured human melanocytes (MC) in relation to immune phenomena, were investigated on ten different MC cell lines from early (1st) to late (22nd) passage. Four melanocyte lines (MC-a) which had undergone changes in growth behaviour during prolonged culture were included in the study, together with two melanoma lines. Cytospin preparations of the cells were stained for the presence of a set of different immunological markers and a melanoma-associated antigen (MAA). All MC lines, including the MC-a and the melanoma lines, showed expression of MHC class I, IL-1, IL-2, ICAM-1 and the MAA, NKI-Beteb, during all passages tested. Interestingly, four of the MC lines showed staining for the Fc receptor. A tendency towards a stronger expression of ICAM-1 on a higher percentage of cells was observed on MC with increasing passage number, the MC-a and the melanoma lines. Expression of the MAA was strongly reduced for the MC-a lines in comparison with the MC and the M14 melanoma lines. Positive staining for the HLA class II molecules was obtained on MC of intermediate and late passages, and on the MC-a and the melanoma lines in the decreasing order HLA-DR, DP and DQ. Additionally, we carried out a preliminary study showing that cultured MC also produce IL-1 and IL-6. However, we were not able to show the production of biologically active IL-2 testing several cultured MC lines. Nevertheless, the overall results taken together suggest that MC are immunologically important cells that are susceptible to changes during long-term culture.
研究了来自早期（第1代）至晚期（第22代）传代的十种不同人黑素细胞（MC）系中不同免疫标志物的表达与免疫现象的关系。该研究纳入了四个在长期培养过程中生长行为发生变化的黑素细胞系（MC-a）以及两个黑色素瘤系。对细胞的甩片标本进行染色，以检测一组不同免疫标志物和一种黑色素瘤相关抗原（MAA）的存在。所有MC系，包括MC-a和黑色素瘤系，在所有测试传代中均显示出MHC I类、IL-1、IL-2、ICAM-1和MAA（NKI-Beteb）的表达。有趣的是，四个MC系显示出Fc受体染色。随着传代数增加，在MC、MC-a和黑色素瘤系中观察到ICAM-1在更高比例细胞上的表达有增强趋势。与MC和M14黑色素瘤系相比，MC-a系中MAA的表达显著降低。在中期和晚期传代的MC以及MC-a和黑色素瘤系上获得了HLA II类分子的阳性染色，按HLA-DR、DP和DQ的顺序递减。此外，我们进行了一项初步研究，表明培养的MC也产生IL-1和IL-6。然而，在检测多个培养的MC系时，我们未能显示出生物活性IL-2的产生。尽管如此，综合总体结果表明，MC是免疫重要细胞，在长期培养过程中易发生变化。
