Nakagawa H, Rhodes A R, Fitzpatrick T B, Hori Y
J Invest Dermatol. 1984 Aug;83(2):140-4. doi: 10.1111/1523-1747.ep12263344.
A cytochemical study of acid phosphatase (AcPase) activity was conducted in normal epidermal melanocytes from both sun-exposed and sun-protected human skin to define the relationship between enzyme activity and melanosome formation. In the perikarya of melanocytes of both sun-exposed and sun-protected skin, it was determined that only a small proportion of stage 1 and 2 melanosomes had AcPase activity (20-33% and 9-26%, respectively). The proportion of AcPase-positive melanosomes in perikarya increased in stage 3 (39-56%), reaching a maximum in stage 4 (67-84%). In the dendrite of melanocytes, where melanosomes were mostly in stages 3 and 4, the vast majority of melanosomes demonstrated AcPase activity (79-87% and 88-93%, respectively). The preferential incorporation of AcPase in the later stages of melanogenesis is more consistent with a possible role for this enzyme in the degradation or transfer of melanosomes, rather than as an essential component in the early process of melanization.
对来自人类暴露于阳光和未暴露于阳光皮肤的正常表皮黑素细胞进行了酸性磷酸酶（AcPase）活性的细胞化学研究，以确定酶活性与黑素小体形成之间的关系。在暴露于阳光和未暴露于阳光皮肤的黑素细胞胞核中，发现只有一小部分1期和2期黑素小体具有AcPase活性（分别为20%-33%和9%-26%）。胞核中AcPase阳性黑素小体的比例在3期增加（39%-56%），在4期达到最高（67%-84%）。在黑素细胞的树突中，黑素小体大多处于3期和4期，绝大多数黑素小体显示出AcPase活性（分别为79%-87%和88%-93%）。AcPase在黑素生成后期的优先掺入，更符合该酶在黑素小体降解或转运中可能发挥的作用，而不是作为黑素化早期过程中的必需成分。
