Ressequie W D, Hughes B L
Poult Sci. 1984 Jul;63(7):1438-43. doi: 10.3382/ps.0631438.
Two experiments were conducted with undiluted chicken semen to determine if fluctuations in endogenous phospholipids and cholesterol might relate to loss of fertilizing capacity which accompanies in vitro storage for 24 hr at 5 C. The following parameters were monitored quantitatively: individual seminal plasma and spermatozoal phospholipids, spermatozoal cholesterol to phospholipid molar ratios, and spermatozoal cholesterol mole percent. After storage for various periods, total lipids were extracted with chloroform:methanol (2:1, v/v). Total phospholipids were obtained by precipitation with cold acetone and further resolved into individual components by thin layer chromatography. All phospholipids and cholesterol were quantitated spectrophotometrically. Spermatozoal phospholipids, the spermatozoal molar ratio of cholesterol to phospholipid, and the spermatozoal mole percent of cholesterol remained essentially the same throughout the 24-hr storage period. Seminal plasma phosphatidylethanolamine also did not fluctuate significantly during the storage period. However, the phosphatidylcholine and the phosphatidylserine-inositol fractions from the seminal plasma appeared to increase with time.
进行了两项使用未稀释鸡精液的实验，以确定内源性磷脂和胆固醇的波动是否可能与受精能力的丧失有关，这种受精能力丧失伴随着在5℃下体外储存24小时。对以下参数进行了定量监测：个体精浆和精子磷脂、精子胆固醇与磷脂的摩尔比以及精子胆固醇摩尔百分比。在储存不同时间段后，用氯仿：甲醇（2:1，v/v）提取总脂质。通过用冷丙酮沉淀获得总磷脂，并通过薄层色谱进一步分离成各个组分。所有磷脂和胆固醇均通过分光光度法定量。在整个24小时储存期内，精子磷脂、精子胆固醇与磷脂的摩尔比以及精子胆固醇摩尔百分比基本保持不变。精浆磷脂酰乙醇胺在储存期内也没有显著波动。然而，精浆中的磷脂酰胆碱和磷脂酰丝氨酸 - 肌醇部分似乎随时间增加。
