Resseguie W D, Hughes B L
Poult Sci. 1984 Aug;63(8):1653-8. doi: 10.3382/ps.0631653.
The possibility of an activation of chicken spermatozoal phospholipase A2 during in vitro storage of undiluted semen was investigated. After storage for periods up to 24 hr at 5 C, the ability of semen to catabolize added phosphatidylcholine labeled on the 2nd carbon of glycerol with 14C oleic acid was assessed. Neither spermatozoa nor plasma from undiluted chicken semen was found to be capable of catabolizing added 14C labeled phosphatidylcholine, regardless of the storage period. No enzyme activity was evident when fresh semen was incubated under various assay conditions to determine the influence of cholic acid, calcium, and the type of phospholipid substrate on phospholipase A2 activity. The influence of spermatozoal protease activation on phospholipase A2 activity from undiluted chicken semen and from Naja naja naja was also investigated and found to have no influence on phospholipase A2 activity.
研究了未稀释鸡精液体外储存期间鸡精子磷脂酶A2被激活的可能性。在5℃储存长达24小时后，评估了精液分解添加的在甘油的第二个碳原子上标记有14C油酸的磷脂酰胆碱的能力。无论储存时间长短，未稀释鸡精液中的精子和血浆均未发现能够分解添加的14C标记的磷脂酰胆碱。当新鲜精液在各种测定条件下孵育以确定胆酸、钙和磷脂底物类型对磷脂酶A2活性的影响时，未发现酶活性。还研究了精子蛋白酶激活对未稀释鸡精液和眼镜蛇毒液中磷脂酶A2活性的影响，发现其对磷脂酶A2活性没有影响。
