Kumar S, McKerlie M L, Albrecht T B, Goldman A S, Baron S
Proc Soc Exp Biol Med. 1984 Oct;177(1):104-11. doi: 10.3181/00379727-177-41918.
To help assess the possibility that a newly described viral inhibitor from cell cultures might play a natural defensive role in vivo, its distribution and concentration in human and animal organ extracts and body fluids were investigated. The concentration of the inhibitor was high in human liver, heart muscle, splenic extracts, and human serum and milk. The inhibitor in the body was indistinguishable from a previously described inhibitor produced in cell cultures that was characterized by broad antiviral activity, lack of target cell species specificity, lack of induction of stable antiviral activity in cells, rapid reversibility of antiviral action, prevention of virus attachment, and stability at 100 degrees C. Sixteen virus plaque reduction units of the inhibitor diminished the yield of poliovirus in vitro by more than 1000-fold. Additional evidence that contact-blocking viral inhibitor (CVI) inhibits vaccinia virus attachment to cells is presented. A role for the inhibitor in natural defense against viral infections is possible.
为了评估一种新描述的来自细胞培养物的病毒抑制剂在体内可能发挥天然防御作用的可能性，研究了其在人和动物器官提取物及体液中的分布和浓度。该抑制剂在人肝脏、心肌、脾脏提取物以及人血清和乳汁中的浓度较高。体内的这种抑制剂与先前在细胞培养物中产生的一种抑制剂无法区分，后者具有广泛的抗病毒活性、缺乏靶细胞种类特异性、不会在细胞中诱导稳定的抗病毒活性、抗病毒作用具有快速可逆性、能阻止病毒附着以及在100摄氏度下稳定等特点。16个病毒蚀斑减少单位的该抑制剂可使脊髓灰质炎病毒在体外的产量降低1000倍以上。还提供了接触阻断病毒抑制剂（CVI）抑制痘苗病毒附着细胞的更多证据。该抑制剂在天然抗病毒感染防御中可能发挥作用。
