Lampidis T J, Salet C, Moreno G, Chen L B
Agents Actions. 1984 Jun;14(5-6):751-7. doi: 10.1007/BF01978920.
Rhodamine 123, a cationic fluorescent dye, has previously been shown to specifically localize in or on mitochondria in living cells. Since it has also been shown to be relatively non-toxic in a variety of cell types it has been a useful tool for probing mitochondria in vitro. In this report, using cardiac cells in culture, we demonstrate that rhodamine 123 and 6G both positively charged compounds, quickly inhibit beating and kill cardiac-muscle cells while uncharged or neutral rhodamines, 116 and B, produce neither effect. We also present data which illustrate that the cationic rhodamines inhibit oxidative phosphorylation in isolated mitochondria while the neutral dyes do not. It is suggested that both phenomena may be related.
罗丹明123是一种阳离子荧光染料，先前已证明它能特异性地定位于活细胞的线粒体内部或表面。由于它在多种细胞类型中也显示出相对无毒，因此它一直是体外探测线粒体的有用工具。在本报告中，我们利用培养的心脏细胞证明，罗丹明123和6G这两种带正电荷的化合物，会迅速抑制心脏跳动并杀死心肌细胞，而不带电荷或呈中性的罗丹明116和B则没有这种作用。我们还提供了数据表明，阳离子罗丹明会抑制分离线粒体中的氧化磷酸化，而中性染料则不会。有人认为这两种现象可能有关联。
