Abe K, Takano H, Ito T
Biol Reprod. 1982 Apr;26(3):501-9. doi: 10.1095/biolreprod26.3.501.
The mouse epididymis was ligated at the junction of the head and body at 20 to 30 days or 90 days of age, and examined by light and electron microscopy 30 days after ligation. In the normal mouse, the lumen of the duct in the corpus epididymidis was distended with abundant spermatozoa and PAS-positive material. In the epididymis ligated at 20 to 30 days, the duct of the corpus was decreased in diameter and contained no spermatozoa and little PAS-positive material in the lumen. Peculiar pale, vacuolated cells appeared scattered or clustered in the epithelium in this region. The cells often formed intercellular cysts. The vacuoles and intercellular cysts had long microvilli on their inner surface. In the epididymis ligated at 90 days, few or no pale cells were seen in the epithelium of the duct. These findings imply that the differentiation of the epithelial cells is dependent on the luminal contents of the epididymal duct.
在20至30日龄或90日龄时，将小鼠附睾在头部与体部的交界处进行结扎，并在结扎后30天通过光学显微镜和电子显微镜进行检查。在正常小鼠中，附睾体部的管腔充满大量精子和PAS阳性物质而扩张。在20至30日龄时结扎的附睾中，附睾体部的管径减小，管腔内无精子且PAS阳性物质很少。在该区域的上皮中可见奇特的淡色、空泡化细胞散在或聚集成团。这些细胞常形成细胞间囊肿。空泡和细胞间囊肿的内表面有长微绒毛。在90日龄时结扎的附睾中，在附睾管的上皮中很少见到或未见淡色细胞。这些发现表明上皮细胞的分化依赖于附睾管的管腔内容物。
