Makarov V L, Dimitrov S I, Tsaneva I R, Pashev I G
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1021-7. doi: 10.1016/0006-291x(84)91193-8.
Calf thymus chromatin was digested with trypsin and the structural alterations which occurred were followed by flow linear dichroism. After a sharp initial increase, the amplitude of the positive signal gradually decreased followed by a change of the sign of the dichroism and further increase of the negative signal up to a plateau. These changes of the dichroism were compared to the respective changes in the histone pattern. It was shown that the positive dichroism of chromatin did not depend on the condensation state of chromatin, and that the orientation of the nucleosomes along the chromatin fiber was maintained by the globular domain of H1 and the non-structured parts of core histones.
用胰蛋白酶消化小牛胸腺染色质，并用流动线性二色性跟踪所发生的结构变化。在最初急剧增加之后，正信号的幅度逐渐减小，随后二色性符号改变，负信号进一步增加直至达到平稳状态。将这些二色性变化与组蛋白模式的相应变化进行比较。结果表明，染色质的正二色性不依赖于染色质的凝聚状态，并且核小体沿染色质纤维的取向由H1的球状结构域和核心组蛋白的非结构化部分维持。
