McLean L R, Phillips M C
Biochim Biophys Acta. 1984 Sep 19;776(1):21-6. doi: 10.1016/0005-2736(84)90246-3.
The rates of transfer of [14C]cholesterol from small and large unilamellar cholesterol/egg yolk phosphatidylcholine vesicles to a common vesicle acceptor were compared at 37 degrees C. The rate of exchange of cholesterol between vesicles of identical cholesterol concentrations (20 mol%) did not differ from the rate of transfer from donor vesicles containing 20 mol% cholesterol to egg yolk PC vesicles. Further, the rate of transfer of [14C]cholesterol from vesicles containing 15 mol% dicetyl phosphate (to confer a negative charge) was not different from the rate of transfer from neutral vesicles. However, the half-time for transfer of [14C]cholesterol from large unilamellar donor vesicles was about 5-times greater (10.2 h, 80 nm diameter) than from small unilamellar vesicles (2.3 h, 23 nm diameter). These data suggest that increased curvature in small unilamellar vesicles reduces cholesterol-nearest neighbor interactions to allow a more rapid transfer of cholesterol into the aqueous phase.
在37摄氏度下，比较了[14C]胆固醇从小单层和大单层胆固醇/蛋黄磷脂酰胆碱囊泡向共同囊泡受体的转移速率。相同胆固醇浓度（20摩尔%）的囊泡之间胆固醇的交换速率与从含有20摩尔%胆固醇的供体囊泡向蛋黄PC囊泡的转移速率没有差异。此外，[14C]胆固醇从含有15摩尔%磷酸二鲸蜡酯（赋予负电荷）的囊泡的转移速率与从中性囊泡的转移速率没有差异。然而，[14C]胆固醇从大单层供体囊泡转移的半衰期（10.2小时，直径80纳米）比从小单层囊泡（2.3小时，直径23纳米）大约大5倍。这些数据表明，小单层囊泡中增加的曲率降低了胆固醇-最近邻相互作用，从而使胆固醇更快地转移到水相中。
