Evanochko W T, Sakai T T, Ng T C, Krishna N R, Kim H D, Zeidler R B, Ghanta V K, Brockman R W, Schiffer L M, Braunschweiger P G
Biochim Biophys Acta. 1984 Sep 14;805(1):104-16. doi: 10.1016/0167-4889(84)90042-9.
Perchloric acid extracts of radiation-induced fibrosarcoma (RIF-1) tumors grown in mice have been analyzed by multinuclear NMR spectroscopy and by various chromatographic methods. This analysis has permitted the unambiguous assignment of the 31P resonances observed in vivo to specific phosphorus-containing metabolites. The region of the in vivo spectra generally assigned to sugar phosphates has been found in RIF-1 tumors to contain primarily phosphorylethanolamine and phosphorylcholine rather than glycolytic intermediates. Phosphocreatine was observed in extracts of these tumor cells grown in culture as well as in the in vivo spectra, indicating that at least some of the phosphocreatine observed in vivo arises from the tumor itself and not from normal tissues. In the 31P-NMR spectra of the perchloric acid extract, resonances originating from purine and pyrimidine nucleoside di- and triphosphate were resolved. HPLC analyses of the nucleotide pool indicate that adenine derivatives were the most abundant components, but other nucleotides were present in significant amounts. The 1H and 13C resonance assignments of the majority of metabolites present in RIF-1 extracts have also been made. Of particular importance is the ability to observe lactate, the levels of which may provide a noninvasive measure of glycolysis in these cells in both the in vitro states. In addition, the aminosulfonic acid, taurine, was found in high levels in the tumor extracts.
通过多核核磁共振光谱法和各种色谱方法，对在小鼠体内生长的辐射诱导纤维肉瘤（RIF - 1）肿瘤的高氯酸提取物进行了分析。该分析使得能够明确将体内观察到的³¹P共振归属于特定的含磷代谢物。在RIF - 1肿瘤中发现，通常归属于糖磷酸酯的体内光谱区域主要含有磷酸乙醇胺和磷酸胆碱，而非糖酵解中间产物。在这些培养的肿瘤细胞提取物以及体内光谱中均观察到了磷酸肌酸，这表明体内观察到的至少部分磷酸肌酸源自肿瘤本身而非正常组织。在高氯酸提取物的³¹P - NMR光谱中，分辨出了源自嘌呤和嘧啶核苷二磷酸及三磷酸的共振。核苷酸库的高效液相色谱分析表明，腺嘌呤衍生物是最丰富的成分，但其他核苷酸也大量存在。还对RIF - 1提取物中存在的大多数代谢物进行了¹H和¹³C共振归属。特别重要的是能够观察到乳酸，其水平可能为体外和体内状态下这些细胞中的糖酵解提供一种非侵入性测量方法。此外，在肿瘤提取物中发现了高含量的氨基磺酸——牛磺酸。
