Antonenkov V D, Panchenko L F
Biokhimiia. 1984 Jul;49(7):1159-65.
Subcellular distribution of NADP+-dependent dehydrogenases of pentose phosphate pathway (glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase) in rat liver was studied, using differential and isopycnic centrifugation. The enzyme activity was detected in the purified peroxisomal fraction as well as in the cytosol. Both dehydrogenases are localized in the peroxisomal matrix. Administration of a hypolipidaemic drug clofibrate increases the amount of both enzymes in peroxisomes. The putative functional role of pentose phosphate cycle dehydrogenases in peroxisomes is discussed.
采用差速离心和等密度离心法，研究了大鼠肝脏中磷酸戊糖途径的NADP⁺依赖性脱氢酶（葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶）的亚细胞分布。在纯化的过氧化物酶体组分以及胞质溶胶中均检测到了酶活性。两种脱氢酶均定位于过氧化物酶体基质中。给予降血脂药物氯贝丁酯可增加过氧化物酶体中这两种酶的含量。文中讨论了过氧化物酶体中磷酸戊糖循环脱氢酶的假定功能作用。
