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豆科根瘤菌3841中发育调控的脂多糖抗原的基因去阻遏

Genetic derepression of a developmentally regulated lipopolysaccharide antigen from Rhizobium leguminosarum 3841.

作者信息

Wood E A, Butcher G W, Brewin N J, Kannenberg E L

机构信息

John Innes Institute, Norwich, United Kingdom.

出版信息

J Bacteriol. 1989 Sep;171(9):4549-55. doi: 10.1128/jb.171.9.4549-4555.1989.

DOI:10.1128/jb.171.9.4549-4555.1989
PMID:2768182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210249/
Abstract

Monoclonal antibody AFRC MAC 203 recognizes a developmentally regulated lipopolysaccharide antigen in Rhizobium leguminosarum bv. viciae 3841. Transposon-induced mutants that constitutively expressed MAC 203 antigen were isolated. These strains were morphologically normal, showed no gross abnormalities in lipopolysaccharide size distribution on sodium dodecyl sulfate-polyacrylamide gels, and induced normal nitrogen-fixing nodules. However, the mutants lacked lipopolysaccharide epitopes recognized by another rat monoclonal antibody, AFRC MAC 281, suggesting that the corresponding epitopes may be interconverted or share a common precursor. In conjugational crosses, the transposon insertion associated with both the loss of MAC 281 antigen and the constitutive expression of MAC 203 antigen showed linkage to the chromosomal rif allele. A derivative of strain 3841 with a deletion spanning the nod-fix region of the symbiotic plasmid showed no altered expression pattern for MAC 203 antigen, suggesting that the relevant genetic determinants map to genomic sites that are not associated with nifA or any known genes on the symbiotic plasmid.

摘要

单克隆抗体AFRC MAC 203识别豌豆根瘤菌蚕豆生物变种3841中一种受发育调控的脂多糖抗原。分离出组成型表达MAC 203抗原的转座子诱导突变体。这些菌株形态正常，在十二烷基硫酸钠-聚丙烯酰胺凝胶上脂多糖大小分布无明显异常，且能诱导正常的固氮根瘤。然而，这些突变体缺乏另一种大鼠单克隆抗体AFRC MAC 281识别的脂多糖表位，这表明相应的表位可能会相互转化或共享一个共同前体。在接合杂交中，与MAC 281抗原缺失和MAC 203抗原组成型表达相关的转座子插入显示与染色体上的利福平抗性等位基因连锁。共生质粒nod-fix区域有缺失的3841菌株衍生物，其MAC 203抗原表达模式未改变，这表明相关的遗传决定因素定位于与共生质粒上的nifA或任何已知基因无关的基因组位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/fd309e1be45d/jbacter00175-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/d20309ce9cee/jbacter00175-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/49a71d10a11d/jbacter00175-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/fd309e1be45d/jbacter00175-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/d20309ce9cee/jbacter00175-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/49a71d10a11d/jbacter00175-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15d/210249/fd309e1be45d/jbacter00175-0038-a.jpg

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本文引用的文献

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Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene.
豌豆根瘤环境恢复了豆科根瘤菌脂多糖acpXL突变体向其脂多糖A添加27-羟基二十八烷酸的能力。
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A Rhizobium leguminosarum AcpXL mutant produces lipopolysaccharide lacking 27-hydroxyoctacosanoic acid.一种豌豆根瘤菌AcpXL突变体产生缺乏27-羟基二十八烷酸的脂多糖。
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Immunolocalization of PsNLEC-1, a lectin-like glycoprotein expressed in developing pea nodules.豌豆根瘤发育过程中表达的一种类凝集素糖蛋白PsNLEC-1的免疫定位
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