Ealick S E, Cook W J, Fontecilla-Camps J C, Suddath F L, Bugg C E, Watt D D
J Biol Chem. 1984 Oct 10;259(19):12081-3.
Crystals of Variant-2 scorpion toxin have been grown using seeding techniques from 30% 2-methyl-2,4-pentanediol at pH 9.2 and T = 4 degrees C. These crystals display a temperature-dependent, reversible phase transition near room temperature. The apparent space group for the high-temperature form is P3121 or P3221 with a = 48.8(1) A and c = 43.7(1) A, and with one molecule per asymmetric unit. At lower temperature, the crystals undergo a phase transition in which the space group remains the same but with c' (approximately equal to 2c) = 86.1(1) A. In addition, the low-temperature form displays several weak, diffuse reflections that correspond to a tripling of the a axis. The high-temperature form diffracts beyond 1.8-A resolution and appears to be suitable for a complete structural study.
利用晶种技术，在pH值为9.2、温度T = 4摄氏度的30% 2-甲基-2,4-戊二醇中培养出了变体2蝎毒素晶体。这些晶体在室温附近呈现出温度依赖性的可逆相变。高温形式的表观空间群为P3121或P3221，a = 48.8(1) Å，c = 43.7(1) Å，每个不对称单元有一个分子。在较低温度下，晶体发生相变，空间群保持不变，但c'（约等于2c）= 86.1(1) Å。此外，低温形式显示出几个较弱的漫反射，对应于a轴的三倍。高温形式的衍射分辨率超过1.8 Å，似乎适合进行完整的结构研究。
