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在感染H-1细小病毒的细胞中鉴定非衣壳细小病毒蛋白NCVP1的多种形式。

Identification of multiple forms of the noncapsid parvovirus protein NCVP1 in H-1 parvovirus-infected cells.

作者信息

Paradiso P R

出版信息

J Virol. 1984 Oct;52(1):82-7. doi: 10.1128/JVI.52.1.82-87.1984.

DOI:10.1128/JVI.52.1.82-87.1984
PMID:6481857
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254492/
Abstract

Analysis of extracts of H-1 parvovirus-infected cells with virus-specific antiserum led to the identification of two forms of the noncapsid virus protein NCVP1. These two proteins had apparent molecular weights of 84,000 (NCVP1) and 92,000 (NCVP1') and were structurally related, based on their immunological reactivity and on peptide map analysis. Both of these proteins appeared early in the virus infection, about the same time that capsid proteins appeared. NCVP1' was a highly phosphorylated protein which was apparently derived from NCVP1 via a post-translational event. Phosphoserine was the predominant phosphorylated amino acid in NCVP1' and appeared to be localized in one site or a few sites on the protein. The possible involvement of these noncapsid proteins in parvovirus DNA replication is discussed.

摘要

用病毒特异性抗血清对H-1细小病毒感染细胞的提取物进行分析，鉴定出非衣壳病毒蛋白NCVP1的两种形式。这两种蛋白的表观分子量分别为84,000（NCVP1）和92,000（NCVP1'），基于它们的免疫反应性和肽图分析，二者在结构上相关。这两种蛋白均在病毒感染早期出现，大约与衣壳蛋白出现的时间相同。NCVP1'是一种高度磷酸化的蛋白，显然是通过翻译后事件从NCVP1衍生而来。磷酸丝氨酸是NCVP1'中主要的磷酸化氨基酸，似乎定位于该蛋白的一个或几个位点上。文中讨论了这些非衣壳蛋白在细小病毒DNA复制中可能的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/649bfff54ba7/jvirol00127-0096-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/9be8e2c8bd01/jvirol00127-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/1c8d64afdfa7/jvirol00127-0093-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/2b58eaa051e9/jvirol00127-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/fe34fd28a82f/jvirol00127-0094-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/6ff906368c78/jvirol00127-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/f83957b4d9f9/jvirol00127-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/f29388dff819/jvirol00127-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/649bfff54ba7/jvirol00127-0096-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/9be8e2c8bd01/jvirol00127-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/1c8d64afdfa7/jvirol00127-0093-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/2b58eaa051e9/jvirol00127-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/fe34fd28a82f/jvirol00127-0094-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/6ff906368c78/jvirol00127-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/f83957b4d9f9/jvirol00127-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/f29388dff819/jvirol00127-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d0/254492/649bfff54ba7/jvirol00127-0096-b.jpg

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