Perry V H, Oehler R, Cowey A
Neuroscience. 1984 Aug;12(4):1101-23. doi: 10.1016/0306-4522(84)90006-x.
Horseradish peroxidase was deposited in the optic nerve to retrogradely label and reveal the dendritic form of all classes of ganglion cell, or it was injected into the dorsal lateral geniculate nucleus to reveal only those classes projecting to the thalamus. The results were compared with those of the accompanying paper in which the ganglion cells projecting to the midbrain are selectively revealed. Two major classes of ganglion cells are described, the P alpha and P beta cells. For both classes dendritic field size increases with eccentricity from the fovea and there is no overlap in the two classes at any given eccentricity. Cell body size shows a similar mean difference but with a slight overlap. Both cell bodies and dendritic fields are larger along the temporal horizontal meridian than the nasal horizontal meridian, for P alpha and for P beta cells, but these differences are reduced when naso-temporal differences in ganglion cell density are taken into account, that is, size correlates closely with density. Injections restricted to the parvocellular layers of the lateral geniculate nucleus labelled almost exclusively P beta cells, whereas injections confined to the magnocellular layers labelled almost exclusively P alpha cells. As midbrain injections label no P beta cells and few P alpha cells it can be shown that about 80% of ganglion cells are P beta cells projecting to parvocellular lateral geniculate nucleus, and that about 10% are P alpha cells projecting to magnocellular layers. The coverage factor, that is the number of cells covering each point on the retina, varied from 1.9-2.3 for P beta cells, and from 2-7 for P alpha cells. Comparing the results with those of comparable investigations on cats and rabbits shows a much clearer segregation of the terminal targets of different classes of ganglion cell in monkeys, the greatest difference being the absence in the monkey of a projection to the geniculate from gamma- and epsilon-like cells. Further, axons which branch and innervate both thalamus and midbrain are rare in monkeys but common in other mammals. Comparing the results with those from physiological investigations suggests that the P beta cells correspond to colour-opponent cells, whereas P alpha cells correspond to the achromatic broad-band magnocellular cells.
将辣根过氧化物酶注入视神经以逆行标记并揭示所有类型神经节细胞的树突形态，或者将其注入背外侧膝状核以仅揭示那些投射到丘脑的细胞类型。将结果与随附论文的结果进行比较，在随附论文中选择性地揭示了投射到中脑的神经节细胞。描述了两类主要的神经节细胞，即Pα细胞和Pβ细胞。对于这两类细胞，树突野大小都随着离中央凹的偏心度增加而增大，并且在任何给定的偏心度下，这两类细胞的树突野都没有重叠。细胞体大小显示出类似的平均差异，但有轻微重叠。对于Pα细胞和Pβ细胞，沿着颞侧水平子午线的细胞体和树突野都比鼻侧水平子午线的大，但是当考虑到神经节细胞密度的鼻颞差异时，这些差异会减小，也就是说，大小与密度密切相关。局限于外侧膝状核小细胞层的注射几乎只标记Pβ细胞，而局限于大细胞层的注射几乎只标记Pα细胞。由于注入中脑几乎不标记Pβ细胞且很少标记Pα细胞，因此可以表明，约80%的神经节细胞是投射到小细胞外侧膝状核的Pβ细胞，约10%是投射到大细胞层的Pα细胞。覆盖因子，即覆盖视网膜上每个点的细胞数量，Pβ细胞为1.9 - 2.3，Pα细胞为2 - 7。将结果与对猫和兔子的类似研究结果进行比较表明，猴子中不同类型神经节细胞的终末靶标分离得更清晰，最大的差异是猴子中没有来自γ样和ε样细胞向膝状体的投射。此外，在猴子中分支并支配丘脑和中脑的轴突很少见，但在其他哺乳动物中很常见。将结果与生理学研究结果进行比较表明，Pβ细胞对应于颜色拮抗细胞，而Pα细胞对应于无色宽带大细胞。
