Kondratenko G P, Kats G L, Poliak E Z, Gnilitskaia V B, Gritsenko L Z
Zh Mikrobiol Epidemiol Immunobiol. 1984 Aug(8):41-4.
In this work the data obtained in the quantitative investigations of sputum samples from 106 miners having acute pneumonia are presented. These investigations were carried out twice at the peak of the disease to determine the possible infective agent. The virological study of nasal impression smears by immunofluorescence and the serological study of paired sera made it possible to establish the viral and bacterial nature of the disease in 12% of cases. The expediency of the quantitative investigations of sputum, carried out twice, in combination with the study of the biological properties of opportunistic microorganisms was shown. Streptococcus pneumoniae proved to play the most important etiological role in the appearance of acute pneumonia in miners. This infective agent was detected in 82% of patients by the inoculation of sputum samples in "diagnostic" dilutions (10(-5) and higher). The associations of pneumococci with staphylococci, hemolytic bacteria and Neisseria were found to be capable of playing a significant role in the development of acute inflammation in pulmonary tissue, especially in those cases when these associations were isolated from highly diluted sputum (10(-5)).
本文展示了对106名患有急性肺炎的矿工痰液样本进行定量研究所得的数据。在疾病高峰期进行了两次此类研究，以确定可能的感染源。通过免疫荧光对鼻印涂片进行病毒学研究以及对双份血清进行血清学研究，在12%的病例中确定了疾病的病毒和细菌性质。研究表明，进行两次痰液定量研究并结合对机会性微生物生物学特性的研究是合理的。肺炎链球菌被证明在矿工急性肺炎的发生中起最重要的病因学作用。通过将痰液样本接种于“诊断”稀释度（10^(-5)及更高），在82%的患者中检测到了这种感染源。发现肺炎球菌与葡萄球菌、溶血性细菌和奈瑟菌的组合能够在肺组织急性炎症的发展中起重要作用，尤其是在那些从高度稀释痰液（10^(-5)）中分离出这些组合的病例中。
