Domke I, Weis W
Ann Nutr Metab. 1984;28(5):261-7. doi: 10.1159/000176815.
Feeding a fat-free diet for 3 weeks decreases the activity of ascorbate:ferricytochrome b5 oxidoreductase and increases NADH:monodehydroascorbate oxidoreductase in rat liver microsomes. Contrary to Vmax, the apparent Km for monodehydroascorbate of the first enzyme was not depressed. The activity of ascorbate:ferricytochrome b5 oxidoreductase is increased by incubation with microsomal lipid extract, thus showing that the loss of activity results from dietary-induced changes of microsomal fatty acid composition.
给大鼠喂食3周无脂饮食会降低抗坏血酸：铁细胞色素b5氧化还原酶的活性，并增加大鼠肝脏微粒体中NADH：单脱氢抗坏血酸氧化还原酶的活性。与最大反应速度（Vmax）相反，第一种酶对单脱氢抗坏血酸的表观米氏常数（Km）并未降低。抗坏血酸：铁细胞色素b5氧化还原酶的活性通过与微粒体脂质提取物孵育而增加，因此表明活性丧失是由饮食诱导的微粒体脂肪酸组成变化引起的。
