Cockroft D L
Br J Exp Pathol. 1984 Oct;65(5):625-36.
Rat embryos explanted at stages between primitive streak and head-fold were exposed in rotating bottle cultures to glucose levels raised by 3-15 mg/ml either throughout a 66-h culture period, or for shorter intervals near the start of culture (with the rest of the culture period at normal glucose levels). A high proportion of embryos explanted at 8 days 20 h or at 9 days and exposed to 12 mg/ml exogenous glucose for only the first or second 8-h periods of a 66-68-h culture were malformed, whereas embryos exposed for the third 8 h were relatively resistant, and embryos exposed later still were unaffected. There were indications that embryos at stages of development corresponding to the second 8-h periods in these cultures were particularly susceptible to hyperglycaemia. Embryos explanted at 9 days exhibited malformations when exposed to 6-9 mg/ml glucose throughout culture, compared with the 12-15 mg/ml shown previously to be necessary to induce anomalies in embryos explanted 12 h later.
将处于原条期至头褶期之间的大鼠胚胎，在旋转瓶培养中暴露于葡萄糖水平升高3 - 15毫克/毫升的环境中，这一过程持续66小时的培养期，或者在培养开始时的较短时间段内进行（培养期的其余时间处于正常葡萄糖水平）。在66 - 68小时培养期的第一个或第二个8小时期间，仅暴露于12毫克/毫升外源性葡萄糖的、在8天20小时或9天取出的胚胎中，有很大比例出现畸形，而在第三个8小时暴露的胚胎相对具有抗性，之后暴露的胚胎则未受影响。有迹象表明，在这些培养中与第二个8小时阶段相对应的发育阶段的胚胎，对高血糖特别敏感。与之前显示的、在12小时后取出的胚胎中诱导异常所需的12 - 15毫克/毫升相比，在9天取出的胚胎在整个培养过程中暴露于6 - 9毫克/毫升葡萄糖时会出现畸形。
