Neill S D, O'Brien J J, McMurray C H, Blanchflower W J
Equine Vet J. 1984 Sep;16(5):430-4. doi: 10.1111/j.2042-3306.1984.tb01966.x.
Cellular fatty acid compositions of contagious equine metritis isolates and three reference Haemophilus equigenitalis cultures were determined by gas chromatography. The chromatographic data were standardised and normalised fatty acid methyl ester (FAME) profiles were produced. The profiles were compared visually and similarity indices were determined using a computer peak matching method. There was little difference between the profiles of the three reference strains, each strain being characterised by three major fatty acids; C 18:1, C 16:0 and 30H-C 14:0. Variations in cultural conditions had no significant effect on the FAME profiles. The identification of laboratory isolates using the technique was in agreement with the presumptive identification based on the currently recommended tests and an improvement on the confirmatory serological identification. The FAME profiles provided confirmation of identity where it was not possible to use the presently recommended serological procedures. The authors recommend the gas chromatography technique for use in the diagnostic laboratory as an adjunct to the presently accepted identification methods.
通过气相色谱法测定了传染性马子宫内膜炎分离株和三株马生殖支原体参考培养物的细胞脂肪酸组成。对色谱数据进行标准化处理，生成了归一化的脂肪酸甲酯（FAME）图谱。通过目视比较图谱，并使用计算机峰匹配方法确定相似性指数。三株参考菌株的图谱之间差异不大，每株菌株均以三种主要脂肪酸为特征；C 18:1、C 16:0和30H-C 14:0。培养条件的变化对FAME图谱没有显著影响。使用该技术对实验室分离株的鉴定结果与基于当前推荐试验的初步鉴定结果一致，并且在确证血清学鉴定方面有所改进。当无法使用当前推荐的血清学程序时，FAME图谱可提供身份确认。作者建议在诊断实验室中使用气相色谱技术作为目前公认的鉴定方法的辅助手段。
