Patel R, Hola M, Riley P A, Wilkie D
Exp Cell Biol. 1984;52(3):176-82. doi: 10.1159/000163258.
Growth of guinea-pig keratocytes (GPK) proceeded normally in standard medium in which glutamine (mitochondrial substrate) replaced glucose as the carbon and energy source. Cells in the glucose-free glutamine cultures were much more sensitive to the toxic and growth-inhibiting effects of the antimitochondrial drugs ethidium bromide, chloramphenicol and oligomycin than cells in glucose-containing cultures. In the latter, cells continued to proliferate in the presence of these drugs after 2-3 days incubation, but in the non-fermenting cells growth was arrested within 24 h and there was more than 95% cell death after 2 days. Cycloheximide, a general inhibitor of protein synthesis in eukaryotic cells, showed no selective inhibition of growth and glutamine and glucose cultures were equally affected. The system appears to be useful for the detection of primary antimitochondrial activity of drugs in general.
豚鼠角膜细胞（GPK）在标准培养基中正常生长，该培养基中谷氨酰胺（线粒体底物）替代葡萄糖作为碳源和能量来源。与含葡萄糖培养的细胞相比，无葡萄糖谷氨酰胺培养的细胞对线粒体药物溴化乙锭、氯霉素和寡霉素的毒性及生长抑制作用更为敏感。在含葡萄糖培养中，细胞在这些药物存在下孵育2 - 3天后仍继续增殖，但在非发酵细胞中，生长在24小时内停止，2天后细胞死亡率超过95%。环己酰亚胺是真核细胞蛋白质合成的一般抑制剂，未表现出对生长的选择性抑制，谷氨酰胺和葡萄糖培养的细胞受到同等影响。总体而言，该系统似乎可用于检测药物的原发性线粒体活性。
