Bosseray N, Plommet A M, Plommet M
Dev Biol Stand. 1984;56:257-70.
Lots of five weeks old female CD-1 mice were vaccinated at several doses with living (B. abortus 19), killed whole cells or purified fractions vaccines. The doses were the number of bacterial cells injected per mouse, either viable (living vaccines), total (killed) or number calculated from the yield of extraction. The mice were intraperitoneally injected with a lyophilized standard challenge, 2 X 10(5) Brucella abortus 544, thirty days after vaccination. In two assays, B. melitensis H.38 was used as challenge at several doses. Responses to a good live vaccine were dose independent. Responses to killed whole cell and purified fractions were usually dose dependent. A reference lyophilized vaccine previously prepared and titrated would give the reference response to one unit. These data can be used to devise a general control method of activity for anti-Brucella vaccines. This was proposed and discussed in the preceding paper.
将多组五周龄雌性CD - 1小鼠用不同剂量的活疫苗（流产布鲁氏菌19株）、死全菌疫苗或纯化组分疫苗进行接种。剂量是指每只小鼠注射的细菌细胞数量，对于活疫苗是活菌数量，对于死疫苗是总菌数，对于纯化组分疫苗是根据提取产量计算出的数量。在接种疫苗30天后，给小鼠腹腔注射冻干的标准攻击菌液，2×10⁵ 流产布鲁氏菌544。在两次试验中，使用不同剂量的羊种布鲁氏菌H.38作为攻击菌。对优质活疫苗的反应与剂量无关。对死全菌疫苗和纯化组分疫苗的反应通常与剂量有关。先前制备并滴定的参考冻干疫苗对一个单位会产生参考反应。这些数据可用于设计抗布鲁氏菌疫苗活性的通用控制方法。这在前一篇论文中已提出并讨论。
