Edwards J A, Brimijoin S
J Neurochem. 1982 May;38(5):1393-403. doi: 10.1111/j.1471-4159.1982.tb07918.x.
Investigating the possibility that acetylcholinesterase (AchE) and butyrylcholinesterase (BuChE) are regulated in a coordinated manner, we have examined the natural variation in activity of these two enzymes in several tissues of adult male Sprague-Dawley, Fischer-344, and Wistar-Furth rats. Both enzymes varied greatly in mean activity among brain, diaphragm, atria, serum, superior cervical ganglia, and liver. In Sprague-Dawley rats there were also large individual variations with up to a fivefold range of AChE activities and up to a 100-fold range of BuChE activities in a given tissue. Individual variations in cholinesterase activities appeared to be smaller in the inbred Fischer-344 or Wistar-Furth rats. Experiments with internal standards of partially purified AChE and BuChE indicated that the individual variations probably reflected differences in the intrinsic content or specific activity of the tissue enzymes. Comparison of the AChE activities in different tissues of a given group of rats failed to reveal statistically significant correlations in any strain (i.e., the relative activity of any one tissue was no guide to the relative activity of any other tissue in the same rat). This result indicates that the regulation of AChE is tissue-specific. By contrast, BuChE activity showed highly significant correlations among the majority of the tissues examined in the Sprague-Dawley rats, implying that widely dispersed factors can affect the regulation of this enzyme. Body-wide regulation is not necessarily the rule, however, since only a single tissue pair in the inbred Fischer rats and none of the pairs in the Wistar-Furth rats showed significant correlations of BuChE activity. In general, AChE and BuChE activities were not correlated with each other to a statistically significant degree. We conclude that the control of these enzymes normally involves different mechanisms and is strongly affected by the genetic background of the sample population.
为了研究乙酰胆碱酯酶（AchE）和丁酰胆碱酯酶（BuChE）是否以协同方式受到调节，我们检测了成年雄性斯普拉格 - 道利大鼠、费希尔344大鼠和威斯塔 - 富思大鼠几种组织中这两种酶活性的自然变异。在脑、膈肌、心房、血清、颈上神经节和肝脏中，这两种酶的平均活性差异很大。在斯普拉格 - 道利大鼠中，特定组织内AChE活性的个体差异也很大，范围可达五倍，BuChE活性范围可达100倍。在近交系费希尔344大鼠或威斯塔 - 富思大鼠中，胆碱酯酶活性的个体差异似乎较小。使用部分纯化的AChE和BuChE内标进行的实验表明，个体差异可能反映了组织酶内在含量或比活性的差异。对给定一组大鼠不同组织中的AChE活性进行比较，未发现任何品系中有统计学上显著的相关性（即同一大鼠中任何一个组织的相对活性无法指导任何其他组织的相对活性）。这一结果表明AChE的调节具有组织特异性。相比之下，在斯普拉格 - 道利大鼠中，大多数检测组织的BuChE活性显示出高度显著的相关性，这意味着广泛分布的因素可以影响该酶的调节。然而，全身调节不一定是规律，因为在近交系费希尔大鼠中只有一对组织，而在威斯塔 - 富思大鼠中没有任何组织对显示出BuChE活性的显著相关性。一般来说，AChE和BuChE活性在统计学上没有显著的相关性。我们得出结论，这些酶的控制通常涉及不同的机制，并受到样本群体遗传背景的强烈影响。
