Hansen S L, Freedy P K
J Clin Microbiol. 1984 Sep;20(3):494-9. doi: 10.1128/jcm.20.3.494-499.1984.
The abilities of commercial MIC, automated, and reference methods for in vitro detection of methicillin-resistant Staphylococcus aureus were determined on 49 strains from eight hospitals. Micro-Media, MicroScan, Sensititre, Sceptor, API Uniscept KB, Abbott MS-2, Vitek AMS, Autobac MTS, NCCLS disk diffusion, and broth microdilution antimicrobial susceptibility testing procedures were evaluated. All testing was performed by using manufacturers' or reference procedures, and results were determined at no later than 24 h of incubation at 35 degrees C. With NCCLS disk diffusion, all strains were resistant to oxacillin (1 microgram), 47 (96%) were resistant to methicillin (4 micrograms), and 48 (98%) were resistant to nafcillin (1 microgram). The percentages of strains resistant to methicillin (greater than 8 micrograms/ml) were 98% with API Uniscept KB, 86% with Sceptor, MicroScan, and Autobac MTS, 84% with Sensititre, 71% with Micro-Media, and 70% with NCCLS MIC. Abbott MS-2 detected 86% of strains resistant to methicillin (greater than 5 micrograms/ml). With oxacillin (greater than 2 micrograms/ml), 90% were detected with Vitek AMS and 70% were detected with NCCLS MIC. With nafcillin (greater than 2 micrograms), 82% were resistant with Micro-Media, 57% were resistant by NCCLS MIC, and 50% (three of six) were resistant by MicroScan. Two strains from one hospital and one strain from another gave susceptible results with all automated and commercial methods. All strains from three centers were detected by all methods. Variability also occurred among the systems with cephalothin, clindamycin, gentamicin, chloramphenicol, and trimethoprim sulfamethoxazole.
采用来自八家医院的49株菌株，对商业微量肉汤稀释法（MIC）、自动化方法及参考方法体外检测耐甲氧西林金黄色葡萄球菌的能力进行了测定。对Micro-Media、MicroScan、Sensititre、Sceptor、API Uniscept KB、雅培MS-2、Vitek AMS、Autobac MTS、美国国家临床实验室标准化委员会（NCCLS）纸片扩散法及肉汤微量稀释抗菌药物敏感性试验程序进行了评估。所有检测均按照制造商或参考程序进行，结果在35℃孵育不超过24小时时判定。采用NCCLS纸片扩散法时，所有菌株对苯唑西林（1微克）耐药，47株（96%）对甲氧西林（4微克）耐药，48株（98%）对萘夫西林（1微克）耐药。对甲氧西林耐药（大于8微克/毫升）的菌株比例，API Uniscept KB为98%，Sceptor、MicroScan及Autobac MTS为86%，Sensititre为84%，Micro-Media为71%，NCCLS MIC为70%。雅培MS-2检测出对甲氧西林耐药（大于5微克/毫升）的菌株为86%。对于苯唑西林（大于2微克/毫升），Vitek AMS检测出90%，NCCLS MIC检测出70%。对于萘夫西林（大于2微克），Micro-Media检测出82%耐药，NCCLS MIC检测出57%耐药，MicroScan检测出50%（6株中的3株）耐药。来自一家医院的2株菌株及另一家医院的1株菌株，所有自动化及商业方法检测结果均为敏感。来自三个中心的所有菌株均能被所有方法检测出。在头孢噻吩、克林霉素、庆大霉素、氯霉素及复方磺胺甲恶唑检测中，各系统之间也存在差异。
