Hashimoto K, Okawa Y, Mikami T, Suzuki S, Suzuki M
J Pharmacobiodyn. 1984 Jul;7(7):472-8. doi: 10.1248/bpb1978.7.472.
An acidic mannan from bakers' yeast designated as WAM025 was investigated for its augmentative effect on cytolytic activity of phagocytes obtained from the peritoneal cavity of C3H/He strain mice, which had been administered i.p. with this acidic polysaccharide, 150 mg/kg/d, 5 and 10 times. The result of assay for the cytolytic effect of WAM025-activated phagocytes by measuring the amounts of 3H-uridine of the MM46 tumor cells indicated that a marked lysis of the target cells took place by a 24-h incubation with the activated phagocytes and that a decrease of this effect was observed by the additions of either phagocytic inhibitors or active oxygen scavengers. Because generation of active oxygens from the WAM025-activated phagocytes was also diminished by the additions of the same inhibitors and scavengers, it indicated that active oxygens participated in an important role in the cytolytic effect displayed by the WAM025-activated phagocytes.
对一种来自面包酵母的酸性甘露聚糖（命名为WAM025）进行了研究，观察其对从C3H/He品系小鼠腹腔获得的吞噬细胞的溶细胞活性的增强作用，这些小鼠经腹腔注射这种酸性多糖，剂量为150 mg/kg/天，分别注射5次和10次。通过测量MM46肿瘤细胞的3H-尿苷量来检测WAM025激活的吞噬细胞的溶细胞作用，结果表明，与激活的吞噬细胞孵育24小时后，靶细胞发生明显裂解，并且加入吞噬抑制剂或活性氧清除剂后，这种作用减弱。由于加入相同的抑制剂和清除剂后，WAM025激活的吞噬细胞的活性氧生成也减少，这表明活性氧在WAM025激活的吞噬细胞所表现出的溶细胞作用中起重要作用。
