Grega D S
J Neurosci Methods. 1984 Aug;11(3):199-203. doi: 10.1016/0165-0270(84)90039-6.
A new method for culturing spinal cord slices or explants is presented which entails the use of a commercially available purified collagen, Vitrogen. Vitrogen provides a stable three-dimensional matrix for culturing spinal cord explants which is superior to the conventional method of applying explants to moist dishes coated with rat tail collagen. The use of Vitrogen facilitated the culturing of spinal cord explants which remain viable for over 2 1/2 weeks in culture, in addition to enhancing neuritic growth.
本文介绍了一种培养脊髓切片或外植体的新方法，该方法需要使用市售的纯化胶原蛋白Vitrogen。Vitrogen为培养脊髓外植体提供了一个稳定的三维基质，优于将外植体应用于涂有大鼠尾胶原的潮湿培养皿的传统方法。Vitrogen的使用不仅促进了脊髓外植体的培养，使其在培养中存活超过2.5周，还增强了神经突生长。
