DeSousa D J, Rouse A A, Smolon W J
Toxicol Appl Pharmacol. 1984 Nov;76(2):234-42. doi: 10.1016/0041-008x(84)90004-8.
Data from 67 six-rabbit eye irritation tests were used to generate two-, three-, and four-rabbit Draize scores. The 15 two-rabbit, 20 three-rabbit, and 15 four-rabbit "subsample" scores for each of the 67 petrochemicals tested were used to establish prediction intervals for the original six-rabbit scores. Prediction interval length shortens with increasing sample size, is widest in the middle portion of the Draize scale, and can be used to select the minimum number of rabbits necessary to satisfy a required level of precision. Also, the ability of each subsample size to correctly classify the test materials according to an in-house irritation classification system was evaluated. Results indicate that subsamples of size 2, 3, 4, and 5 were 88, 93, 95%, and 96% accurate, respectively (compared to six rabbits), at correctly classifying the irritation potential of the materials tested.
来自67项六兔眼刺激试验的数据被用于生成两兔、三兔和四兔德雷兹评分。对所测试的67种石化产品中的每一种，其15个两兔、20个三兔和15个四兔“子样本”评分被用于建立原始六兔评分的预测区间。预测区间长度随样本量增加而缩短，在德雷兹量表的中间部分最宽，并且可用于选择满足所需精度水平所需的最少兔子数量。此外，还评估了每个子样本量根据内部刺激分类系统正确分类测试材料的能力。结果表明，2只、3只、4只和5只兔子的子样本在正确分类所测试材料的刺激潜力方面的准确率分别为88%、93%、95%和96%（与六兔相比）。
