串珠镰刀菌分离株在鼠伤寒沙门氏菌试验中的致突变活性。
Mutagenic activity of Fusarium moniliforme isolates in the Salmonella typhimurium assay.
作者信息
Bjeldanes L F, Thomson S V
出版信息
Appl Environ Microbiol. 1979 Jun;37(6):1118-21. doi: 10.1128/aem.37.6.1118-1121.1979.
Abstract
A total of 33 isolates of Fusarium moniliforme from several food or feed crops were grown on sterile cracked corn, and chloroform-isopropanol extracts were assayed for mutagenic activity in the Salmonella typhimurium-microsome system by using tester strain TA98 or TA100 or both. Extracts of 21 (64%) of the isolates assayed against TA100 were mutagenic. Activities of seven of these extracts were increased markedly with incorporation of the liver homogenate (S-9) into the assay. Seven (33%) of the isolates assayed against TA98 were weakly active, with the liver homogenate having little effect on reversion rates.
摘要
从几种粮食或饲料作物中总共分离出33株串珠镰刀菌，将其接种在无菌碎玉米上培养，然后用氯仿 - 异丙醇提取物，通过使用测试菌株TA98或TA100或两者，在鼠伤寒沙门氏菌 - 微粒体系统中测定其致突变活性。针对TA100检测的分离物提取物中，有21株（64%）具有致突变性。其中七种提取物的活性在测定中加入肝脏匀浆（S - 9）后显著增加。针对TA98检测的分离物中有七株（33%）活性较弱，肝脏匀浆对回复突变率影响很小。
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