Israel D A, Barry W H, Edell D J, Mark R G
Am J Physiol. 1984 Oct;247(4 Pt 2):H669-74. doi: 10.1152/ajpheart.1984.247.4.H669.
An array of extracellular microelectrodes containing 25 recording and 6 stimulating electrodes was fabricated using microelectronics technology. Ventricular myocardial cells from 8- to 10-day chick embryos were cultured on the surface of the microelectrode array. Extracellular potentials were recorded simultaneously from multiple sites. Simultaneous recordings of extracellular and transmembrane potentials were made from single sites. Extracellular potentials were also recorded simultaneously with cell motion from single sites. Cells were paced by means of electrical stimuli applied via the stimulating electrodes. Conduction velocity in a strip of cells varied linearly as a function of temperature from 0.21 m/s at 26 degrees C to 0.38 m/s at 36.5 degrees C.
利用微电子技术制作了一个包含25个记录电极和6个刺激电极的细胞外微电极阵列。将8至10日龄鸡胚的心室心肌细胞培养在微电极阵列表面。从多个位点同时记录细胞外电位。从单个位点同时记录细胞外电位和跨膜电位。还从单个位点同时记录细胞外电位与细胞运动情况。通过刺激电极施加电刺激对细胞进行起搏。一条细胞带中的传导速度随温度呈线性变化，从26摄氏度时的0.21米/秒变化到36.5摄氏度时的0.38米/秒。
