Denyer M C, Riehle M, Britland S T, Offenhauser A
Centere for Cell Engineering, University of Glasgow, UK.
Med Biol Eng Comput. 1998 Sep;36(5):638-44. doi: 10.1007/BF02524437.
There are a range of techniques that can be used to assay bioactive compound. One potentially promising technique is a system consisting of microfabricated extracellular recording devices over which electrogenic cells can be grown. To date, research in this area has concentrated on the use of neurons as an electrogenic cell type. However, these cells have limitations. Only small extracellular potentials have been recorded from mammalian neurons cultured over microfabricated electrode arrays. Although such potentials may be of use in assays examining the effects of bio-active compound analogues on firing frequency, they are of little use for more detailed pharmacological studies involving analyses of signal shape. What is required is a system from which much larger extracellular potentials can be recorded. This preliminary study reports on a system based on cardiac myocytes cultured over microfabricated metal microelectrode arrays, from which potentials with a mean amplitude of 16.9 microV can be reliably recorded, which can be reversibly blocked with mumoll-1 concentrations of the sodium ion channel blocker lidocaine. Less common potentials with amplitudes of up to 3.5 mV were also recorded. It is demonstrated that cardiac myocytes cultured over microfabricated micro-electrode arrays can be used in assays of cardioactive compound analogues.
有一系列技术可用于分析生物活性化合物。一种潜在有前景的技术是一个由微制造的细胞外记录装置组成的系统，在该装置上可以培养电生细胞。迄今为止，该领域的研究主要集中在将神经元用作电生细胞类型。然而，这些细胞存在局限性。在微制造的电极阵列上培养的哺乳动物神经元仅记录到了小的细胞外电位。尽管这种电位可能在检测生物活性化合物类似物对放电频率的影响的分析中有用，但它们对于涉及信号形状分析的更详细的药理学研究几乎没有用处。需要的是一个能够记录更大细胞外电位的系统。这项初步研究报告了一种基于在微制造的金属微电极阵列上培养心肌细胞的系统，从中可以可靠地记录平均幅度为16.9微伏的电位，该电位可以被微摩尔每升浓度的钠离子通道阻滞剂利多卡因可逆地阻断。还记录到了幅度高达3.5毫伏的不太常见的电位。结果表明，在微制造的微电极阵列上培养的心肌细胞可用于心脏活性化合物类似物的分析。
