Sato K
Am J Physiol. 1984 Oct;247(4 Pt 2):R646-9. doi: 10.1152/ajpregu.1984.247.4.R646.
Transepithelial potential difference (PD) of isolated segments of the secretory coil of both human control and cystic fibrosis (CF) eccrine sweat glands was measured during stimulation with methacholine (MCh) and isoproterenol (ISO) in vitro. Negative luminal PD of about 10 and 16 mV developed across the secretory coil epithelium in control and CF secretory coil, respectively, during maximal MCh stimulation. ISO (10(-5) M) stimulation generated a mean of 1.9-mV negative luminal PD in control glands; however, ISO failed to enhance the luminal PD beyond the resting level of 0.9 mV in the CF secretory coil. The inhibition of ISO-induced (10(-5) M) luminal PD in control secretory coil by propranolol (5 X 10(-5) M) but not by atropine (10(-5) M) indicated its pharmacologic specificity. The possibility is discussed that this lack of electrical driving force during ISO stimulation could be one of the mechanisms for the absence of sweating response to beta-adrenergic stimulation in CF sweat glands reported recently (8). The mechanism for the higher MCh-induced luminal PD in the CF secretory coil remains to be studied.
在体外使用乙酰甲胆碱（MCh）和异丙肾上腺素（ISO）刺激时，测量了人类对照和囊性纤维化（CF）外分泌汗腺分泌盘孤立节段的跨上皮电位差（PD）。在最大MCh刺激期间，对照和CF分泌盘的分泌盘上皮细胞分别产生了约10 mV和16 mV的负腔内PD。ISO（10⁻⁵ M）刺激在对照腺体中产生了平均1.9 mV的负腔内PD；然而，ISO未能使CF分泌盘的腔内PD超过静息水平0.9 mV。普萘洛尔（5×10⁻⁵ M）而非阿托品（10⁻⁵ M）对对照分泌盘中ISO诱导（10⁻⁵ M）的腔内PD的抑制表明了其药理特异性。讨论了ISO刺激期间缺乏电驱动力可能是最近报道的CF汗腺对β-肾上腺素能刺激无出汗反应的机制之一（8）。CF分泌盘中MCh诱导的较高腔内PD的机制仍有待研究。
