Sato K, Sato F
Am J Physiol. 1982 May;242(5):C360-5. doi: 10.1152/ajpcell.1982.242.5.C360.
Transepithelial electrical potential difference (PD) of the secretory portion of the rhesus monkey palm eccrine sweat gland was studied in both Ca2+-containing (control and Ca2+-free Sr2+ media. In the control medium, the luminal PD was a saturation function of the methacholine (MCH) concentration, reaching a plateau at about -6 mV (lumen negative) at 10(-5) M MCH. In the Sr2+ medium, the luminal PD increased spontaneously with a time course similar to that the spontaneous sweat secretion (SSS). Like SSS, the spontaneous luminal PD in the Sr2+ medium is a saturation function of the Sr2+ concentration in the bath, reaching a plateau at -9 mV at 8 mM Sr2+. Addition of MCH to the Sr2+ medium caused a rapid increase in the lumen-negative PD as seen in the control medium. The spontaneous lumen-negative PD was abolished by subsequent addition of Ca2+ to the bath, as was the SSS [K. Sato, Am. J. Physiol. 239 (Cell Physiol. 8): C90-C97, 1980]. The data further reinforce the notion that Sr2+-induced SSS represents true secretory processes which are triggered by Sr2+ leaking into the cell bypassing the receptor-agonist interaction.
在含Ca2+（对照）和无Ca2+的Sr2+培养基中，研究了恒河猴手掌小汗腺分泌部的跨上皮电位差（PD）。在对照培养基中，管腔PD是乙酰甲胆碱（MCH）浓度的饱和函数，在10(-5) M MCH时达到约-6 mV（管腔为负）的平台期。在Sr2+培养基中，管腔PD随时间自发增加，其时间进程与自发汗液分泌（SSS）相似。与SSS一样，Sr2+培养基中的自发管腔PD是浴液中Sr2+浓度的饱和函数，在8 mM Sr2+时达到-9 mV的平台期。向Sr2+培养基中添加MCH会导致管腔负PD迅速增加，这与对照培养基中所见情况相同。随后向浴液中添加Ca2+可消除自发的管腔负PD，SSS也会被消除[K. 佐藤，《美国生理学杂志》239（细胞生理学8）：C90 - C97，1980]。这些数据进一步强化了这样一种观点，即Sr2+诱导的SSS代表了真正的分泌过程，该过程是由Sr2+绕过受体 - 激动剂相互作用泄漏到细胞中所触发的。
