Sabri M I
Arch Toxicol. 1984 Sep;55(3):191-4. doi: 10.1007/BF00316127.
The in vitro and in vivo effect of aliphatic diketones has been studied on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) D,L-glyceraldehyde-3-phosphate: NAD oxidoreductase (phosphorylating EC 1.2.1.12 activity). Neurotoxic diketone, 2,5-hexanedione (2,5-HD), but not 2,4-hexanedione (2,4-HD), a non-neurotoxic diketone, inhibited GAPDH in rat bran homogenate preincubated with 25 mM diketones for 20 min. If the preincubation period was increased to 2 h, approximately 25% and 55% inhibition of GAPDH activity was observed with 1 mM and 5 mM 2,5-HD respectively. The inhibition of GAPDH activity was also seen in sciatic nerves but not in the brain or liver homogenates of rats chronically intoxicated with 2,5-HD for 12 weeks. The inhibition of GAPDH by 2,5-HD appears to be selective, and thus confirms earlier data from this laboratory.
已研究了脂肪族二酮对甘油醛-3-磷酸脱氢酶（GAPDH）D，L-甘油醛-3-磷酸：NAD氧化还原酶（磷酸化，EC 1.2.1.12活性）的体外和体内作用。神经毒性二酮2,5-己二酮（2,5-HD），而非非神经毒性二酮2,4-己二酮（2,4-HD），在与25 mM二酮预孵育20分钟的大鼠脑匀浆中抑制了GAPDH。如果预孵育时间增加到2小时，分别用1 mM和5 mM 2,5-HD观察到GAPDH活性约有25%和55%的抑制。在坐骨神经中也观察到了GAPDH活性的抑制，但在长期用2,5-HD中毒12周的大鼠的脑或肝匀浆中未观察到。2,5-HD对GAPDH的抑制似乎具有选择性，从而证实了本实验室早期的数据。
