Cannas M, Amedeo M R, Denicolai F
Boll Soc Ital Biol Sper. 1984 Aug 31;60(8):1521-5.
The Authors expose their experience about a new model for testing the biocompatibility of materials of possible use as implants in surgery. This model uses a glycoprotein, Fibronectin (FN) involved in cellular adhesion; FN was purified from human plasma by affinity chromatography on denatured collagen; the protein obtained by this procedure is about 95% pure. The best value of FN binding was found to be on bioglass B5 whereas in the case of Titanium Alloy and B6 an intermediate value was measured. Results may be discussed considering other preliminary results about cell adhesion.
作者们阐述了他们关于一种新模型的经验，该模型用于测试可能用作外科植入物的材料的生物相容性。这种模型使用一种参与细胞黏附的糖蛋白，纤连蛋白（FN）；FN通过在变性胶原上的亲和色谱从人血浆中纯化得到；通过该程序获得的蛋白质纯度约为95%。发现FN结合的最佳值出现在生物玻璃B5上，而在钛合金和B6的情况下测得的是中间值。可以结合其他关于细胞黏附的初步结果来讨论这些结果。
