Maurer A M, Han Z C, Dhermy D, Brière J
INSERM U 409, Hôpital Beaujon, Clichy, France.
Leuk Res. 1994 Nov;18(11):837-42. doi: 10.1016/0145-2126(94)90164-3.
We have previously shown that heparin and heparan sulfate stimulate the growth of human erythroleukemia cells in vitro in the presence of serum or plasma. To determine whether heparin and other glycosaminoglycans (GAGs) are involved in the growth of leukemia cells, effects of GAGs on the growth of three leukemia cell lines expressing different phenotypes, the HEL, HL60 and U937 cell lines were studied using both plasma clot and serum-free agar systems. The cells were cultured with different doses of six GAGs: hyaluronic acid, chondroitin sulfate, dermatan sulfate, keratan sulfate, heparin and heparan sulfate. It was found that, in serum-free agar system, no GAG was able to stimulate (HEL) cell growth. In contrast, when serum-containing culture systems were used, all six GAGs promoted colony formation of HL60 and U937 cells. In addition, all GAGs, except keratan sulfate, stimulated the growth of HEL cells. The findings suggest that the GAGs may play an indirect role in enhancing leukemia cell proliferation by different mechanisms.
我们之前已经表明，在血清或血浆存在的情况下，肝素和硫酸乙酰肝素可在体外刺激人红白血病细胞的生长。为了确定肝素和其他糖胺聚糖（GAGs）是否参与白血病细胞的生长，我们使用血浆凝块和无血清琼脂系统研究了GAGs对三种表达不同表型的白血病细胞系（HEL、HL60和U937细胞系）生长的影响。用六种不同剂量的GAGs培养细胞：透明质酸、硫酸软骨素、硫酸皮肤素、硫酸角质素、肝素和硫酸乙酰肝素。结果发现，在无血清琼脂系统中，没有GAG能够刺激（HEL）细胞生长。相反，当使用含血清的培养系统时，所有六种GAGs都促进了HL60和U937细胞的集落形成。此外，除硫酸角质素外，所有GAGs都刺激了HEL细胞的生长。这些发现表明，GAGs可能通过不同机制在增强白血病细胞增殖中起间接作用。
