Källander C F, Simonsson B, Hagberg H, Gronowitz J S
Cancer. 1984 Dec 1;54(11):2450-5. doi: 10.1002/1097-0142(19841201)54:11<2450::aid-cncr2820541123>3.0.co;2-r.
A recently developed deoxythymidine kinase assay, utilizing iodine-125-iodo-deoxyuridine as substrate and capable of detecting enzyme activity in serum from healthy humans, was used in an investigation of sera from 55 untreated patients with chronic lymphocytic leukemia (CLL). When confined to the study, the patients were classified as having progressive or indolent disease and according to Rai stage. The results showed a significant correlation between serum deoxythymidine kinase activity (S-TK) and disease status, i.e., higher values were found in patients with progressive disease, compared to those with indolent disease. S-TK also correlated with Rai stage. S-TK values of more than 40 times the normal value were found in some patients. All patients with S-TK greater than 8.4 units had a disease that was or became progressive during the observation period. Within the patient group with indolent disease two groups that differed with regard to prognosis could be distinguished according to their initial S-TK values. In longitudinal studies of 18 patients with indolent disease, S-TK was found to exceed 8.4 units only on one occasion during an observation period of up to 68 months. In patients with indolent disease, a transition to progressive disease was parallelled by an increase in S-TK. Studies of S-TK levels in 18 patients receiving treatment showed that S-TK decreased during successful therapy. S-TK was also found to increase when the disease was reactivated. From these results it is concluded that S-TK could be used as a prognostic marker for the individual CLL patient. Furthermore, S-TK seems to be useful for longitudinal follow-up studies of disease status, both in indolent disease and in progressive disease during treatment.
一种最近开发的脱氧胸苷激酶检测方法，以碘-125-碘脱氧尿苷为底物，能够检测健康人血清中的酶活性，被用于对55例未经治疗的慢性淋巴细胞白血病（CLL）患者的血清进行研究。在该研究中，患者被分类为患有进展性或惰性疾病，并根据Rai分期进行分类。结果显示血清脱氧胸苷激酶活性（S-TK）与疾病状态之间存在显著相关性，即与惰性疾病患者相比，进展性疾病患者的S-TK值更高。S-TK也与Rai分期相关。在一些患者中发现S-TK值超过正常值的40倍。所有S-TK大于8.4单位的患者在观察期内疾病处于进展期或病情进展。在惰性疾病患者组中，根据其初始S-TK值可区分出预后不同的两组。在对18例惰性疾病患者的纵向研究中，在长达68个月的观察期内，仅在一次检测中发现S-TK超过8.4单位。在惰性疾病患者中，向进展性疾病的转变伴随着S-TK的升高。对18例接受治疗患者的S-TK水平研究表明，在成功治疗期间S-TK降低。当疾病复发时，S-TK也会升高。从这些结果可以得出结论，S-TK可作为个体CLL患者的预后标志物。此外，S-TK似乎对疾病状态的纵向随访研究有用，无论是在惰性疾病还是在治疗期间的进展性疾病中。
