Hille A, Rosa P, Huttner W B
FEBS Lett. 1984 Nov 5;177(1):129-34. doi: 10.1016/0014-5793(84)80996-5.
Protein sulfation was studied in germ-free rats by prolonged in vivo labeling with [35S]sulfate. Specific sets of sulfated proteins were observed in all tissues examined, in leucocytes, and in blood plasma. No protein sulfation was detected in erythrocytes. Analysis of the type of sulfate linkage showed that sulfated proteins secreted into the plasma contained predominantly tyrosine sulfate, whereas sulfated proteins found in tissues contained largely carbohydrate sulfate. This implies some kind of selection concerning the intracellular processing, secretion, turnover or re-uptake of sulfated proteins which is responsible for the enrichment of tyrosine-sulfated proteins in the plasma.
通过用[35S]硫酸盐进行长时间体内标记，对无菌大鼠的蛋白质硫酸化进行了研究。在所有检测的组织、白细胞和血浆中均观察到特定的硫酸化蛋白质组。在红细胞中未检测到蛋白质硫酸化。对硫酸酯键类型的分析表明，分泌到血浆中的硫酸化蛋白质主要含有酪氨酸硫酸盐，而在组织中发现的硫酸化蛋白质主要含有碳水化合物硫酸盐。这意味着在硫酸化蛋白质的细胞内加工、分泌、周转或再摄取方面存在某种选择，这导致血浆中酪氨酸硫酸化蛋白质的富集。
