Colquhoun E Q, Thomson R M
FEBS Lett. 1984 Nov 19;177(2):221-6. doi: 10.1016/0014-5793(84)81287-9.
Lysosomes prepared from rat liver and kidney after loading with the detergent Triton WR-1339 show membrane-bound 5'-deiodinase activity with marked specificity for 3,3',5'-triiodothyronine (reverse T3), lesser activity with respect to thyroxine (T4) and almost none towards 3,3',5'-triiodothyronine (T3). The enzyme is thiol dependent and shows maximal catalysis at pH 7.2. As many of the states known to alter thyroid hormone levels also affect lysosomal function, inhibition of the lysosomal 5'-deiodinase leading to an increase in intracellular reverse T3 may be an initiating mechanism for thyroid hormone change.
用去污剂曲拉通WR - 1339装载后从大鼠肝脏和肾脏制备的溶酶体显示出膜结合的5'-脱碘酶活性，对3,3',5'-三碘甲状腺原氨酸（反式T3）具有显著特异性，对甲状腺素（T4）的活性较低，对3,3',5'-三碘甲状腺原氨酸（T3）几乎没有活性。该酶依赖巯基，在pH 7.2时表现出最大催化活性。由于许多已知会改变甲状腺激素水平的状态也会影响溶酶体功能，溶酶体5'-脱碘酶的抑制导致细胞内反式T3增加可能是甲状腺激素变化的起始机制。
