Wall V W, Kreger A S, Richardson S H
Infect Immun. 1984 Dec;46(3):773-7. doi: 10.1128/iai.46.3.773-777.1984.
Conditions are described for the production of an extracellular cytotoxin or CHO cell-killing factor by Vibrio fluvialis, a recently recognized enteric pathogen. The cell-killing factor was ammonium sulfate precipitable, heat labile, and inactivated by proteases, and had an isoelectric point (estimated by sucrose density gradient electrofocusing) and an apparent molecular weight (estimated by gel filtration) of ca. 4.8 and 12,200, respectively. The culture supernatant fluids also possessed hemolytic and phospholipase A2 activities; however, they were separable from cell-killing factor activity by gel filtration. The substance(s) possessing the hemolytic and phospholipase activities coeluted when fractionated by gel filtration with Sephacryl S-200 (apparent molecular weight of ca. 34,500) and had isoelectric points of ca. 4.4 and 4.5, respectively.
本文描述了由河流弧菌（一种最近被确认的肠道病原体）产生细胞外细胞毒素或CHO细胞杀伤因子的条件。细胞杀伤因子可被硫酸铵沉淀，对热不稳定，可被蛋白酶灭活，其等电点（通过蔗糖密度梯度电聚焦估算）和约为4.8，表观分子量（通过凝胶过滤估算）约为12,200。培养上清液还具有溶血和磷脂酶A2活性；然而，通过凝胶过滤可将它们与细胞杀伤因子活性分离。具有溶血和磷脂酶活性的物质在使用Sephacryl S - 200进行凝胶过滤分级分离时共洗脱（表观分子量约为34,500），其等电点分别约为4.4和4.5。
