Oliver J D, Warner R A, Cleland D R
Appl Environ Microbiol. 1982 Dec;44(6):1404-14. doi: 10.1128/aem.44.6.1404-1414.1982.
Water, sediment, plankton, and animal samples from five coastal sites from North Carolina to Georgia were sampled for their lactose-fermenting vibrio populations. Over 20% of all vibrios tested were sucrose negative and o-nitrophenyl-beta-D-galactopyranoside (ONPG) positive, suggesting identification as the human pathogen Vibrio vulnificus. These vibrios were isolated from all sample sites and sources (water, sediment, plankton, and animals). Correlations with several of 19 environmental parameters monitored at each site were found for total vibrios. The presence of ONPG-positive, sucrose-negative vibrios was correlated with hydrocarbon levels in the water and, in the case of plankton samples, with salinity. A total of 279 sucrose-negative, ONPG-positive isolates were subjected to numerical taxonomic analysis, which resulted in three major clusters. Cluster I corresponded to and included 11 reference strains of V. vulnificus. Cluster II contained the largest number (133) of isolates, of which the great majority were bioluminescent. Although having a resemblance to V. harveyi, the isolates were ONPG positive and many were H2S positive. Cluster III consisted of strains similar to the group F vibrios (V. fluvialis). Of all of the isolates, 55% were luminescent, of which over 20% were lethal when injected into mice. Problems involved in detecting lactose fermentation among marine vibrios and the potential pathogenicity of these organisms are discussed.
对从北卡罗来纳州到佐治亚州的五个沿海地点采集的水、沉积物、浮游生物和动物样本进行了乳糖发酵弧菌种群的采样。所有测试弧菌中超过20%蔗糖阴性且β-D-半乳糖吡喃糖苷邻硝基苯酯（ONPG）阳性，表明鉴定为人类病原体创伤弧菌。这些弧菌从所有样本地点和来源（水、沉积物、浮游生物和动物）中分离得到。发现每个地点监测的19个环境参数中的几个与总弧菌存在相关性。ONPG阳性、蔗糖阴性弧菌的存在与水中的碳氢化合物水平相关，对于浮游生物样本而言，与盐度相关。总共279株蔗糖阴性、ONPG阳性分离株进行了数值分类分析，结果形成了三个主要聚类。聚类I对应并包括11株创伤弧菌参考菌株。聚类II包含数量最多（133株）的分离株，其中绝大多数具有生物发光性。尽管与哈维弧菌相似，但这些分离株ONPG阳性且许多H2S阳性。聚类III由类似于F群弧菌（河流弧菌）的菌株组成。所有分离株中，55%具有发光性，其中超过20%注射到小鼠体内时具有致死性。讨论了检测海洋弧菌中乳糖发酵所涉及的问题以及这些生物体的潜在致病性。
