Wigand R, Kümel G
J Gen Virol. 1978 May;39(2):281-92. doi: 10.1099/0022-1317-39-2-281.
The absence from the medium of any of the 13 amino acids essential for cell growth has an inhibiting effect on the multiplication of adenovirus 9-15 and adenovirus I in HeLa cell cultures. The inhibition is accentuated by previous amino acid starvation of the cultures. Whereas with arginine deprivation, the arginine pool inside the cells is at a minimum within 30 min, the cells are assumed to adapt slowly to the new metabolic state, which is characterized by an increased 'turnover' of protein synthesis. With arginine deficiency and in Hanks' BSS some synthesis of virus and capsid proteins takes place. Quantitative and possibly qualitative differences between the influence of the various deficient media were observed. The experiments rule out DNA synthesis as a primary cause of the amino acid deficiency effect. They lead to the hypothesis that arginine deficiency inhibits the formation of an essential protein which is synthesized very late in the infectious cycle under complete MEM.
细胞生长所必需的13种氨基酸中任何一种从培养基中缺失，都会对HeLa细胞培养物中腺病毒9 - 15和腺病毒I的增殖产生抑制作用。培养物先前的氨基酸饥饿会加剧这种抑制作用。在缺乏精氨酸的情况下，细胞内的精氨酸池在30分钟内降至最低，细胞被认为会缓慢适应新的代谢状态，其特征是蛋白质合成的“周转率”增加。在缺乏精氨酸且处于汉克斯平衡盐溶液（Hanks' BSS）中时，会发生一些病毒和衣壳蛋白的合成。观察到了各种缺乏培养基的影响在数量上甚至可能在质量上的差异。这些实验排除了DNA合成是氨基酸缺乏效应的主要原因。它们引出了这样一个假说，即精氨酸缺乏会抑制一种必需蛋白质的形成，这种蛋白质在完全MEM（Minimum Essential Medium，最低必需培养基）条件下的感染周期后期才会合成。
