Conacher H B, Chadha R K, Lawrence J F, Charbonneau S M, Bryce F
Lipids. 1984 Sep;19(9):637-42. doi: 10.1007/BF02534522.
To assess possible incorporation of halogenated fatty acids into the neonate via the milk, a 4-day study was carried out in which lactating Wistar rats were orally dosed with either brominated olive oil (BOO) (0.6 g/kg body wt/day) or chlorinated olive oil (COO) (0.4 g/kg body wt/day) for the first 4 days. On days 1-5 inclusive 2 pups per litter were sacrificed and the stomach curd and livers analyzed for halogenated fatty acids by gas liquid chromatography (GLC). On day 5 all dams also were sacrificed and their livers and adipose tissue similarly analyzed. With BOO, brominated fatty acids (bfa) accumulated in both the milk lipids and neonate liver lipids, and appeared to plateau on day 4 at levels of 2% and 5% respectively. In contrast to the BOO in which approximately 100% of the bfa was dibromostearic (DBS), the milk bfa comprised 79% (DBS), 9% dibromopalmitic (DBP) and 12% dibromomyristic (DBM) acids, suggesting maternal metabolism to the shorter chain brominated acids. In the neonate liver lipids the bfa composition was 47% (DBS), 12% (DBP) and 41% (DBM), suggesting either further metabolism in the neonate and/or preferential accumulation of the shorter chain brominated acids. The analysis of maternal tissue indicated very low bfa residues, contrary to previous studies in non-lactating rats. Similar results were obtained with COO.
为评估卤化脂肪酸是否可能通过乳汁进入新生儿体内，进行了一项为期4天的研究，在该研究中，对哺乳期的Wistar大鼠在头4天口服给予溴化橄榄油（BOO）（0.6克/千克体重/天）或氯化橄榄油（COO）（0.4克/千克体重/天）。在第1至5天（含第5天），每窝处死2只幼崽，对胃凝乳和肝脏进行气相色谱法（GLC）分析以检测卤化脂肪酸。在第5天，所有母鼠也被处死，对其肝脏和脂肪组织进行类似分析。给予BOO后，溴化脂肪酸（bfa）在乳汁脂质和新生鼠肝脏脂质中均有积累，且在第4天似乎达到稳定水平，分别为2%和5%。与BOO中约100%的bfa为二溴硬脂酸（DBS）不同，乳汁中的bfa包括79%（DBS）、9%二溴棕榈酸（DBP）和12%二溴肉豆蔻酸（DBM），这表明母体将其代谢为较短链的溴化酸。在新生鼠肝脏脂质中，bfa的组成是47%（DBS）、12%（DBP）和41%（DBM），这表明要么在新生鼠体内进一步代谢，要么较短链的溴化酸优先积累。母体组织分析表明bfa残留量非常低，这与之前对非哺乳期大鼠的研究结果相反。给予COO也得到了类似的结果。
