Borg M, Kirk D, Baumgarten H, Rüchel R
Sabouraudia. 1984;22(5):357-67. doi: 10.1080/00362178485380611.
A colorimetric assay for the quantitation of microbial cytotoxicity has been developed using cells from a monocyte-like human cell line (U937), epithelial cells (Hela), and fibroblast-like cells (Vero) as targets. The fraction of surviving cells was determined by their content of the dye neutral red which is retained only by live cells and can be quantitated photometrically after controlled lysis. The neutral red retention assay was at least as sensitive as the 51Cr-release assay; it was considerably less laborious, faster, and avoided handling of radioactivity. Among the different Candida species tested, the highest cytotoxicity was associated with C. albicans and C. tropicalis; a lower degree of cytotoxicity was exhibited by C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, and C. pseudotropicalis. Among the strains of a given fungal species cytotoxicity varied by up to 40%.
已开发出一种比色测定法，用于定量微生物细胞毒性，该方法以单核细胞样人细胞系（U937）、上皮细胞（Hela）和成纤维细胞样细胞（Vero）作为靶细胞。存活细胞的比例通过其对染料中性红的摄取量来确定，中性红仅被活细胞保留，在经过控制裂解后可通过光度法进行定量。中性红保留试验的灵敏度至少与51Cr释放试验相同；它的操作要简便得多，速度更快，且避免了放射性物质的处理。在测试的不同念珠菌物种中，白色念珠菌和热带念珠菌的细胞毒性最高；光滑念珠菌、季也蒙念珠菌、克鲁斯念珠菌、近平滑念珠菌和伪热带念珠菌的细胞毒性较低。在给定真菌物种的菌株中，细胞毒性的差异高达40%。
