Borg M, Rüchel R
Department of Medical Microbiology, University of Göttingen, Federal Republic of Germany.
Infect Immun. 1988 Mar;56(3):626-31. doi: 10.1128/iai.56.3.626-631.1988.
We traced an acid proteinase from Candida spp. in the initial stages of the pathogenesis of the mycosis. On infection of human buccal mucosa, proteinase antigens were detected by immuno-scanning electron microscopy on the surface of adhering blastoconidia and invading filamentous cells of C. albicans serotype A. Proteinase antigens were also present on blastoconidia of C. albicans serotype B, but were missing on filamentous cells of this serotype. Proteolytic isolates of C. tropicalis behaved like C. albicans serotype A. An isolate of C. parapsilosis did not express the proteinase antigen under conditions of this study. After infection of mucosa, culture medium of C. albicans or C. tropicalis showed a time-dependent accumulation of acid proteolytic activity, indicating that the visualized antigens represent active proteinase. No such activity was detected in the medium of C. parapsilosis. Preliminary experiments with the proteinase inhibitor pepstatin A revealed an 89% reduction of mucosal adherence of C. albicans (serotype A). These results suggest that Candida proteinase is involved in fungal attachment. The pattern of adherence reflects the differential expression of secretory proteinase by different candidal strains.
我们在真菌病发病机制的初始阶段追踪了念珠菌属的一种酸性蛋白酶。在人类口腔黏膜感染时，通过免疫扫描电子显微镜在A型白色念珠菌黏附的芽生孢子表面和侵入的丝状细胞表面检测到蛋白酶抗原。蛋白酶抗原也存在于B型白色念珠菌的芽生孢子上，但在该血清型的丝状细胞上缺失。热带念珠菌的蛋白水解分离株表现得与A型白色念珠菌相似。近平滑念珠菌的一个分离株在本研究条件下未表达蛋白酶抗原。黏膜感染后，白色念珠菌或热带念珠菌的培养基显示酸性蛋白水解活性呈时间依赖性积累，表明可视化的抗原代表活性蛋白酶。在近平滑念珠菌的培养基中未检测到这种活性。用蛋白酶抑制剂胃蛋白酶抑制剂A进行的初步实验显示，A型白色念珠菌的黏膜黏附减少了89%。这些结果表明念珠菌蛋白酶参与真菌附着。黏附模式反映了不同念珠菌菌株分泌蛋白酶的差异表达。
