Parquet-Gernez A, Mazurier C, Amiral J, Martinoli J L
Thromb Res. 1984 Sep 15;35(6):703-12. doi: 10.1016/0049-3848(84)90273-1.
An Enzyme Linked immuno Sorbent Assay (ELISA) was developed for the measurement of factor IX:Ag. The results obtained in 90 control subjects, 56 hemophilia B patients and 40 patients under oral anticoagulant treatment were compared with factor IX:C and factor IX:Ag levels according to Electro Immuno-Assay (EIA). In healthy volunteers the mean factor IX:C level was 106.7 U/dl, the mean factor IX:Ag level was 96.8 U/dl according to EIA procedure and 101.5 U/dl according to ELISA method. Using ELISA, 10 hemophiliacs had no detectable antigen, and 13 had minute amounts of IX:Ag. All these patients are classified as B-. Among the others 21 had reduced antigen and are considered as BR and 12 had normal level of IX:Ag and are B+. Patients taking oral anticoagulants had not only a decreased factor IX:C level but also a reduced factor IX:Ag level which is always lower when using EIA procedure in the presence of EDTA than according to ELISA method.
开发了一种酶联免疫吸附测定法（ELISA）用于测量凝血因子IX：抗原。将90名对照受试者、56名B型血友病患者和40名接受口服抗凝治疗的患者的检测结果，与采用电免疫测定法（EIA）测得的凝血因子IX：C和凝血因子IX：抗原水平进行比较。在健康志愿者中，根据EIA程序，凝血因子IX：C的平均水平为106.7 U/dl，凝血因子IX：抗原的平均水平为96.8 U/dl；根据ELISA方法，凝血因子IX：抗原的平均水平为101.5 U/dl。使用ELISA法检测时，10名血友病患者检测不到抗原，13名患者有微量的IX：抗原。所有这些患者被归类为B-型。其他患者中，21名患者抗原减少，被认为是BR型，12名患者IX：抗原水平正常，为B+型。服用口服抗凝剂的患者不仅凝血因子IX：C水平降低，凝血因子IX：抗原水平也降低，在存在乙二胺四乙酸（EDTA）的情况下，使用EIA程序检测时其水平总是低于ELISA方法检测的结果。
