Porter G A, Laurent G, Maldague P, Tulkens P
Toxicol Lett. 1984 Nov;23(2):205-13. doi: 10.1016/0378-4274(84)90128-0.
We have performed combined in vivo and in vitro measurements of thymidine uptake into kidney cortex DNA of animals treated with gentamicin for 7 days at 10 or 20 mg/kg daily (BID). Labelled thymidine is taken up by cortex fragments in vitro (90 min incubation) and incorporated into DNA; treatment of the animals with gentamicin results in a significant dose-dependent enhancement of this in vitro thymidine incorporation; labelled cells are found primarily in the proximal tubules and interstitium; there is an excellent correlation (r : 0.983, n = 15) between the changes of incorporation measured in vivo and in vitro as demonstrated by the sequential use of [3H]thymidine (in vivo) and [14C]thymidine (in vitro) within the same animals.
我们对每天以10或20毫克/千克剂量（每日两次）用庆大霉素治疗7天的动物，进行了肾脏皮质DNA中胸苷摄取的体内和体外联合测量。标记的胸苷在体外（孵育90分钟）被皮质片段摄取并掺入DNA；用庆大霉素治疗动物会导致这种体外胸苷掺入的显著剂量依赖性增强；标记细胞主要存在于近端小管和间质中；在同一动物中依次使用[3H]胸苷（体内）和[14C]胸苷（体外），结果表明体内和体外测量的掺入变化之间存在极好的相关性（r = 0.983，n = 15）。
