Rock G, Tocchi M, Ganz P R, Tackaberry E S
Transfusion. 1984 Nov-Dec;24(6):493-8. doi: 10.1046/j.1537-2995.1984.24685066808.x.
The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics not using DEHP, the collection and storage of red cells is still done in DEHP plasticized packs, and in fact the storage life for red cells has recently been increased up to 49 days using new anticoagulant-preservative solutions. We examined the relationship between DEHP and stored red cells. We found that 28 percent of available 14C-DEHP binds immediately to sites in both the membrane and cytosol fractions of the red cells, and that the total amount and distribution of 14C-DEHP does not change significantly over 7 days. When red cell concentrates were stored with or without DEHP, using either plastic (polyolefin) bags not containing DEHP or glass, definite reduction in the osmotic stability of the red cells was found in the absence of DEHP. Plasma-free hemoglobin levels were 90.3 mg per dl after 35 days of storage in plastic packs containing DEHP and 181.7 mg per dl in the polyolefin bags. The advantages of improved in vitro stability of red cells stored in plastics containing DEHP must be weighed against the potential hazards of patient exposure to DEHP.
柔性塑料血袋的发展使得有效的血液成分生产和治疗成为可能。然而，增塑剂邻苯二甲酸二（2-乙基己基）酯（DEHP），其对人体的毒性仍不明确，已知会从塑料中渗出到储存的血液中。尽管有不使用DEHP的塑料制成的袋子，但红细胞的采集和储存仍在使用DEHP增塑的包装中进行，事实上，使用新的抗凝保存溶液，红细胞的储存期最近已延长至49天。我们研究了DEHP与储存红细胞之间的关系。我们发现，可用的14C-DEHP中有28%立即与红细胞膜和胞质部分的位点结合，并且14C-DEHP的总量和分布在7天内没有显著变化。当红细胞浓缩物在有或没有DEHP的情况下储存时，使用不含DEHP的塑料（聚烯烃）袋或玻璃瓶，在没有DEHP的情况下发现红细胞的渗透稳定性明显降低。在含有DEHP的塑料包装中储存35天后，无血浆血红蛋白水平为每分升90.3毫克，在聚烯烃袋中为每分升181.7毫克。必须权衡在含有DEHP的塑料中储存的红细胞体外稳定性提高的优点与患者接触DEHP的潜在危害。
