Burrin J M, Price C P
Ann Clin Biochem. 1984 Sep;21 ( Pt 5):411-6. doi: 10.1177/000456328402100513.
Collection of blood spots on filter paper offers a practical alternative for home monitoring of diabetic patients. We have compared the merits of three protein precipitants, trichloracetic acid (TCA), perchloric acid (PCA) and sulphosalicylic acid (SSA) for the elution of glucose from the filter paper, and their subsequent effects on three enzymic methods, glucose dehydrogenase (GDH), hexokinase (HK), and glucose oxidase (GOD) for the determination of glucose using a microcentrifugal analyser. The combination of TCA elutant with the GDH method was superior with respect to time course of reaction and elution time from the filter paper, and was chosen for routine use. Within- and between-batch precision for this method was 2.7% and 3.2% respectively at normal glucose concentrations. Recovery of glucose added to whole blood was 110 +/- 5%. Comparison with an automated glucose oxidase method for plasma glucose gave a slope of 1.1, intercept of -0.7 and a correlation coefficient of 0.9 (n = 64). We conclude that the combination of TCA and glucose dehydrogenase provides a robust, precise and accurate method for the quantitation of glucose in filter-paper blood spots. The procedure offers increased sensitivity and better precision than GOD methods. The use of TCA as elutant gives a faster elution time and has the least effect on any of the enzymic methods.
收集滤纸上的血斑为糖尿病患者的家庭监测提供了一种实用的替代方法。我们比较了三种蛋白质沉淀剂，即三氯乙酸（TCA）、高氯酸（PCA）和磺基水杨酸（SSA）从滤纸上洗脱葡萄糖的优点，以及它们随后对三种酶法（葡萄糖脱氢酶（GDH）、己糖激酶（HK）和葡萄糖氧化酶（GOD））使用微量离心分析仪测定葡萄糖的影响。TCA洗脱剂与GDH方法的组合在反应时间进程和从滤纸上的洗脱时间方面表现更优，因此被选用于常规检测。该方法在正常葡萄糖浓度下的批内和批间精密度分别为2.7%和3.2%。添加到全血中的葡萄糖回收率为110±5%。与血浆葡萄糖的自动葡萄糖氧化酶法相比，斜率为1.1，截距为-0.7，相关系数为0.9（n = 64）。我们得出结论，TCA和葡萄糖脱氢酶的组合为定量滤纸上血斑中的葡萄糖提供了一种可靠、精确且准确的方法。该方法比GOD方法具有更高的灵敏度和更好的精密度。使用TCA作为洗脱剂可缩短洗脱时间，并且对任何酶法的影响最小。
