Abyholm A S
Scand J Clin Lab Invest. 1981 May;41(3):269-74. doi: 10.3109/00365518109092044.
A method for glucose determination in blood sampled by the patients outside hospital, is described and evaluated. Glucose in capillary blood spotted on filter paper (Schleicher& Schüll No. 2992), dried and stored at room conditions, is stable for at least 7 days. 6.5 mm discs, containing 10.3 microliter capillary blood are punched out from the spots and eluted in percloric acid. The glucose concentration in the eluate is determined by the glucose dehydrogenase method. Using duplicates, CV within assay is 3.6% between assay 4.2%. Mean recovery is 98.3%, SD 9.6%. Correlation to ordinary whole blood glucose dehydrogenase method is good, r = 0.984 (n = 244). The influence of blood spot variation, punching procedure, paper humidity and haematocrit has been examined. Results are compared to those of others, and advantages of the method are discussed.
本文描述并评估了一种用于测定院外患者采集血样中葡萄糖的方法。点在滤纸（施莱歇尔&舒尔2992号）上、干燥后于室温条件下保存的毛细血管血中的葡萄糖至少7天内稳定。从血斑中冲出含10.3微升毛细血管血的6.5毫米圆盘，并用高氯酸洗脱。洗脱液中的葡萄糖浓度通过葡萄糖脱氢酶法测定。采用双份样本，批内变异系数为3.6%，批间为4.2%。平均回收率为98.3%，标准差为9.6%。与普通全血葡萄糖脱氢酶法的相关性良好，r = 0.984（n = 244）。已研究血斑变异、打孔程序、纸张湿度和血细胞比容的影响。将结果与其他方法的结果进行比较，并讨论了该方法的优点。
